The EXO-NET™ technology consists of a novel, covalently linked multilayered three-dimensional matrix (referred to as a Molecular NET) comprising a range of exosome-specific antibodies, along with linkers and spacers. Placement and spacing of the antibodies, linker molecules and spacer molecules confers a characteristic topology on the Molecular NET surface, a characteristic density within each layer, and a characteristic porosity. The porosity is a result of the choice, and method of incorporation, of the component molecules. The pores allow for the size exclusion functions of the EXO-NETs and the antibodies provide affinity binding.
The unique 3-dimensional matrix is applied to magnetic beads, allowing for fast, accurate and highly specific and efficient capture and isolation of exosomes from any liquid biopsy sample. The technology is highly scalable and is compatible with existing automated testing systems making it ideal for high volume clinical laboratory applications.
The EXO-NET™ matrix has the following features that make it a unique universal technology to facilitate the development of a range of liquid biopsy applications: