Edible plant-derived exosome-like nanoparticles (EPDELNs) have been described as having similarities in size, structure and function to mammalian extracellular vesicles. These plant-derived nanovesicles contain bioactive cargo that includes proteins, lipids, and regulatory microRNAs (miRNAs) [1-3]. Interestingly, several studies have suggested that EPDELNs are taken up by the mammalian gastrointestinal tract where they are involved in plant-animal intercellular communication [1-6]. MiRNAs have a role in post-transcriptional gene regulation in both animals and plants, and are known to regulate important biological processes such as immune responses, proliferation and metabolism. In plants, miRNAs are proposed to emerge mostly through duplication of pre-existing miRNAs, which can arise from protein-coding genes and transposable elements as well as spontaneous mutations from hairpin structures. However, it is unknown whether there is a unique profile of the origination for miRNAs that are selectively packaged into EPDELNs. To investigate this, miRNA libraries from kiwifruit were analysed to compare the genomic originations of miRNAs that are present in the fruit EPDELNs [3] and the whole fruit. Preliminary analysis of published kiwifruit plant-derived nanoparticle miRNAs reveal that among a total of 418 characterised miRNAs, 37 are uniquely mapped to the genome reference while 116 were multiply mapped. Of those uniquely mapped, many are involved in plant gene silencing and cellular response pathways.